Study of the Characteristics of Bacteria Isolated from the Pelus River, Banyumas Regency

Authors

  • Mohammad Nurhafid Aquaculture Study Program, Faculty of Fisheries and Marine Sciences, Jenderal Soedirman University St.Dr.Soeparno, Karangwangkal, Purwokerto 53122, Central Java, Indonesia. Author https://orcid.org/0000-0002-7648-6751
  • Marthyana Indyaswari Magister of Biology, Faculty of Biology, Jenderal Soedirman University. St DR. Soeparno No. 63, North Purwokerto, Banyumas Regency, 53122, Central Java, Indonesia. Author
  • Fajar Husen Magister of Biology, Faculty of Biology, Jenderal Soedirman University. St DR. Soeparno No. 63, North Purwokerto, Banyumas Regency, 53122, Central Java, Indonesia. Author
  • Reza Muhammad Riady Aquaculture Study Program, Faculty of Fisheries and Marine Sciences, Jenderal Soedirman University St.Dr.Soeparno, Karangwangkal, Purwokerto 53122, Central Java, Indonesia. Author https://orcid.org/0000-0001-9140-8738

DOI:

https://doi.org/10.62521/6wsyrt43

Keywords:

Enzyme activity, Bacterial communities, Microbial ecology

Abstract

Community activities around the Pelus River cause pollution which affects the bacterial community found in the river. The aim of this research was to determine the characteristics of bacteria isolated from the Pelus river environment in Banyumas Regency. The method used in this research is an exploratory method where bacterial sampling is carried out at 3 different stations. The samples taken were then isolated, observed colony morphology, as well as biochemical and physiological characterization of the bacteria. The research results obtained 10 different isolates. A total of 10 isolates were obtained based on morphological differences. The characteristics of most of the Pelus River bacteria show variations ranging from enzymatic to environmental resistance which describes the waters of the Pelus River. Bacteria isolated from the water have characteristics that identify them as bacteria capable of degrading compounds

Downloads

Download data is not yet available.

References

Palupi, M. (2022). Isolation and Characterization of Proteolytic Bacteria from the Waters of the Mina Padi Cultivation System. Jurnal Lemuru. 4(2), 46–56. https://doi.org/10.36526/lemuru.v4i2.2084

Ayilara, M. S., & Babalola, O. O. (2023) Bioremediation of environmental wastes: the role of microorganisms. Frontiers in Agronomy, 5, 1183691. https://doi.org/10.3389/fagro.2023.1183691

Breijyeh, Z., Jubeh, B., & Karaman, R. (2020). Resistance of Gram-Negative Bacteria to Current Antibacterial Agents and Approaches to Resolve It. Molecules, 25(6), 1340. https://doi.org/10.3390/molecules25061340

Cabral, J. P. (2010). Water microbiology. Bacterial pathogens and water. International journal of environmental research and public health, 7(10), 3657–3703. https://doi.org/10.3390/ijerph7103657

Damayanti, S. C., Komala, O., Effendi, E. M. (2018). Identification of bacteria from liquid organic fertilizer contained in cow rumen. Ecologia: Scientific Journal of Basic and Environmental Sciences, 18(2), 63-71. http://dx.doi.org/10.33751/ekol.v18i2.1627

Fitri, L., & Yasmin, Y. (2011). Isolation and observation of the morphology of chitinolytic bacterial colonies. Scientific Journal of Biology Education, 3(2), 20-25. https://core.ac.uk/download/pdf/297708324.pdf

Garde, S., Chodisetti, P. K., & Reddy, M. (2021). Peptidoglycan: Structure, Synthesis, and Regulation. EcoSal Plus, 9(2). https://doi.org/10.1128/ecosalplus.esp-0010-2020

Indrayani, I., Putra, R. P., Hambali, A., & Ardiansyah, A. (2022). Isolation and characterization of extremophile bacteria for hydrolytic enzyme production from Waepella Hot Spring, Sinjai, Indonesia. Biodiversitas Journal of Biological Diversity, 23(12), 6345-6351. https://doi.org/10.13057/biodiv/d231231

Jackson, C. R., Tyler, H. L., & Millar, J. J. (2013). Determination of microbial extracellular enzyme activity in waters, soils, and sediments using high throughput microplate assays. Journal of visualized experiments, (80), 50399. https://doi.org/10.3791/50399

Joutey, N. T., Bahafid, W., Sayel, H., & El Ghachtouli, N. (2013). Biodegradation: involved microorganisms and genetically engineered microorganisms. Biodegradation-life of science, 1, 289-320. https://doi.org/10.5772/56194

Lay, B. W. (1994). Analisis Mikroba di Laboratorium. Rajawali Pers. Jakarta.

Lukmanulhakim, R. C., Hidayati, N. V., & Baedowi, M. (2023). Analisis Kandungan Logam Berat Kadmium (Cd) dan Kromium (Cr) pada Matriks Air di Sungai Pelus Kabupaten Banyumas, Jawa Tengah. MAIYAH, 2(1), 41-50. https://doi.org/10.20884/1.maiyah.2023.2.1.8295

Meng, S., Peng, T., Liu, X., Wang, H., Huang, T., Gu, J. D., & Hu, Z. (2022). Ecological Role of Bacteria Involved in the Biogeochemical Cycles of Mangroves Based on Functional Genes Detected through GeoChip 5.0. mSphere, 7(1), e0093621. https://doi.org/10.1128/msphere.00936-21

Nugrayani, D., Hidayati, N. V., Muslih, M., Cahyo, T. N., Putri, A. A., Putri, A. N., Ummah, A. N., & Santoso, F. S. (2023). Ecological Risk Potential Of Heavy Metals (Cd, Cr, Fe) In Sediment Of The Pelus River Around The Kauman Batik Home Industry Sokaraja, Banyumas. Jurnal Perikanan Unram, 13(3), 796-805. http://doi.org/10.29303/jp.v13i3.625

Nurhafid, M., Syakuri, H., Oedjijono, O., Listiowati, E., Ekasanti, A., Nugrayani, D., & Pramono, H. (2021). Isolation and Molecular Identification of Proteolytic Bacteria from the Digestive Tract of Tilapia (Oreochromis niloticus) Cultivated in Banyumas Regency. Jurnal Perikanan Universitas Gadjah Mada, 23(2), 95-105. https://doi.org/10.22146/jfs.64072.

Pelczar, M. J., Chan, E. C. S. (1986). Dasar- Dasar Mikrobiologi. UI Press. Jakarta.

Quintin, M., Dukovski, I., Bhatnagar, J., & Segrè, D. (2021). Optimality of extracellular enzyme production and activity in dynamic flux balance modeling. BioRxiv, 1-34. https://doi.org/10.1101/2021.11.01.466736

Rani, I. M., Lestari, P. R., Rahmayani, D. E., Asan, M., Astriani, M. (2017). Test of phosphate solubilizing and IAA producing bacteria on MOL bintaro (Cerbera manghas L.). Journal of Biology and Learning, 4(2), 11-2. http://doi.org/10.25273/florea.v4i2.1752

Sousa, A. M., Machado, I., Nicolau, A., & Pereira, M. O. (2013). Improvements on colony morphology identification towards bacterial profiling. Journal of Microbiological Methods, 95(3), 327–335. https://doi.org/10.1016/j.mimet.2013.09.020

Thatoi, H., Behera, B.C., Mishra, R.R. et al. Biodiversity and biotechnological potential of microorganisms from mangrove ecosystems: a review. Ann Microbiol, 63, 1–19 (2013). https://doi.org/10.1007/s13213-012-0442-7

Yogafanny, E. (2015). The influence of residents' activities on the river border on the water quality of the Winongo River. Journal of Environmental Science & Technology, 7(1), 29-40. https://doi.org/10.20885/jstl.vol7.iss1.art3

cover

Downloads

Published

2024-05-02

Issue

Section

Articles

How to Cite

Study of the Characteristics of Bacteria Isolated from the Pelus River, Banyumas Regency. (2024). Journal Of Artha Biological Engineering, 1(1), 12-20. https://doi.org/10.62521/6wsyrt43