Screening of proteolytic bacteria from the intestines of Tilapia Fish (Oreochromis niloticus) in the mina padi area of Panembangan Village, Cilongok.
DOI:
https://doi.org/10.62521/pm6jjq40Keywords:
Bacteria, Tilapia, Proteolytic activity , Rice cultivationAbstract
Tilapia is a commodity cultivated in the rice-fish farming area of Panembangan, Banyumas. Information related to the presence of potential bacteria in the intestines of fish cultivated in rice-fish areas is still low, especially proteolytic bacteria. The purpose of this study was to determine the proportion and activity index of proteolytic bacteria in the intestines of fish. This study used an observation method with a purposive sampling technique. Proteolytic bacteria were isolated from the intestines of tilapia in blocks A and E. The bacteria that were successfully isolated were grown on 2% skim milk agar media with an incubation time of 48 hours and observed at 24-hour intervals. Based on the results of the study, the proportion of proteolytic bacteria in the intestines of tilapia from both blocks was 28% in block A and 44% in block E, respectively. The proteolytic activity index of both blocks had an average of 0.48 for block A and 0.75 for block E. The highest proteolytic activity index was from block A, namely 0.83, and in block E 3.0. Meanwhile, the smallest proteolytic index is 0.17 block E and 0.22 block A.
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Table 1. Morphological Characteristics of Proteolytic Bacterial Colonies
Isolate
Shape
Elevation
Edge
Colour
Gram KOH
Catalase
Oxidase
Characteristics of Proteolytic Bacterial Isolates Block A
F1S2
Irregular
Convex
Entire
Creamy white
-
-
-
F1S15
Irregular
Raised
Lobate
Yellow
-
+
+
F1S16
Circular
Pulvinate
Entire
Thick white
-
-
+
F1S17
Circular
Flat
Entire
Yellow
-
+
+
F1S19
Filamentous
Flat
Filamentous
Clear white
-
+
+
F1S20
Irregular
Convex
Lobate
Clear white
-
-
+
F1S22
Circular
Umbonate
Filamentous
Clear white
-
-
+
Characteristics of Proteolytic Bacterial Isolates Block E
F5N1
Circular
Flat
Entire
Milky white
-
+
+
F5N2
Circular
Convex
Entire
Cream white
+
-
+
F5N4
Circular
Flat
Entire
Dark cream white
+
-
+
F5N5
Punctiform
Flat
Entire
Clear white
+
-
+
F5N8
Circular
Pulvinate
Entire
Cream/Brown
-
-
+
F5N9
Spindle
Convex
Entire
Cream
+
-
+
F5N11
Irregular
Flat
Undulate
Clear white
+
-
-
F5N18
Circular
Pulvinate
Entire
Cream white
+
-
+
F5N19
Circular
Convex
Entire
Dark white
+
-
+
F5N20
Circular
Raised
Entire
Cream white
+
-
+
F5N22
Circular
Flat
Entire
Cream white
+
-
-
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