Screening of Amylolytic Bacteria from the Intestines of Tilapia (Oreochromis niloticus) Cultivated in Rice Fish Farming in Panembangan Village, Cilongok District, Banyumas
DOI:
https://doi.org/10.62521/p5vczh22Keywords:
Rice Fish Farming, Tilapia, Fish Intestines, Amylolytic Bacteria, Proportion, Amylolytic Activity IndexAbstract
Amylolytic bacteria in the digestive tract of tilapia are bacteria that produce
amylase enzymes, which function to break down carbohydrates, thereby aiding
the digestive process. The presence of amylolytic bacteria is influenced by feed
and the environment. The purpose of this study was to determine the proportion
and activity index of amylolytic bacteria in the intestines of tilapia. This study
used an observational method with purposive sampling. Amylolytic bacteria
were isolated from the intestines of tilapia in Rice fish farming Blocks A and E.
The successfully isolated bacteria were grown on 1% starch agar medium with
an incubation time of 48 hours and observed at 24-hour intervals. Then, several
characteristic tests were carried out, including Gram staining, KOH, catalase,
and oxidase tests. Based on the results of the study, the proportion of amylolytic
bacteria was found in 10 isolates with a proportion of 44% and 12 isolates with
a proportion of 48% in the intestines of tilapia from the two blocks. The average
amylolytic activity index obtained from block A was 0.96 ± 0.237, and from
block E was 1.49 ± 0.501. The largest amylolytic activity index was obtained
in isolate KSA 19 with a value of 1.33 from block A, while from block E, the
largest amylolytic activity index value was 2.6 in isolate KSE 5.
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